Composite

Part:BBa_K2928015:Design

Designed by: Lauren Hartz   Group: iGEM19_ASIJ_Tokyo   (2019-10-16)


Alpha Amylase (malS) with RBS and Anderson promoter J23101


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1276
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 42
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 412
    Illegal AgeI site found at 1577
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to find a promoter of appropriate strength, after various experimentation we settled on the one above.


Source

The parts making up this composite part are all from previously existing parts on the registry.

References